5-BROMO-4-CHLORO-3-INDOLYL beta-GALACTOSIDE

Synonyms. 5-Bromo-4-chloro-3-indolyl beta-galactoside; BCIG; X-Gal; 5-Bromo-4-chloroindol-3-yl beta-D-galactopyranoside; 5-bromo-4-chloro-1H-indol-3-yl beta-D-Galactopyranoside; 5-Bromo-4-chloro-3-(beta-D-galactopyranosyloxy)indole; (2S,3R,4S,5R,6R)-2- ((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6- (hydroxymethyl) oxane-3,4,5-triol; (2S,4S,3R,5R,6R)-2-(B-bromo-4-chloroindol-3-yloxy)-6- (hydroxymethyl)-2H-3,4,5,6-tetrahydropyran- 3,4,5-triol;

5-BROMO-4-CHLORO-3-INDOLYL BETA-GALACTOSIDE

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PRODUCT IDENTIFICATION

CAS RN

7240-90-6

EINECS RN

230-640-8

FORMULA

 C14H15BrClNO6

MOLE WEIGHT

408.63

H.S CODE

 2940.00.6000

SMILES

 c1(c2c(c[nH]c2ccc1Br)O[C@H]1[C@@H]([C@H]([C@H]([C@H] (O1) CO)O) O) O) Cl

CLASSIFICATION

Chromogenic Compound, Indicator, Galactoside

EXTRA NOTES

A chromogenic substrate for beta-galactosidase.

 

PHYSICAL AND CHEMICAL PROPERTIES

PHYSICAL STATE

white crystalline powder

MELTING POINT

230 C

BOILING POINT

 

DENSITY

 

SOLUBILITY IN WATER

 

SOLVENT SOLUBILITY

 

VAPOR DENSITY

 

log P(octanol-water)

  

VAPOR PRESSURE

  

AUTOIGNITION TEMP

  
pK

 

REFRACTIVE INDEX

 

FLASH POINT

 

 

STABILITY AND REACTIVITY
STABILITY Stable under normal conditions.

INCOMPATIBLE MATERIALS

Strong oxidizing agents.

POLYMERIZATION

Has not been reported

NFPA RATINGS

Health: 1, Flammability: 1, Reactivity: 0

 

EXTERNAL LINKS & GENERAL DESCRIPTION

USA.gov - 5-Bromo-4-chloro-3-indolyl beta-galactoside

Wikipedia Linking - X-gal

Google Scholar Search - 5-Bromo-4-chloro-3-indolyl beta-galactoside

U.S. National Library of Medicine - 5-Bromo-4-chloro-3-indolyl beta-galactoside

PubChem Compound Summary - 5-Bromo-4-chloro-3-indolyl beta-galactoside

NCBI (http://www.ncbi.nlm.nih.gov/) -  5-Bromo-4-chloro-3-indolyl beta-galactoside

Material Safety Data Sheet - 5-Bromo-4-chloro-3-indolyl beta-galactoside

Human Metabolome Database - 5-Bromo-4-chloro-3-indolyl beta-galactoside

http://www.sigmaaldrich.com
5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for b-galactosidase.1 X-Gal is cleaved by b-galactosidase to yield an insoluble blue precipitate. X-Gal is particularly useful in molecular biology applications to detect the activity of b-galactosidase which is frequently used as a reporter gene. In cloning, X-Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using a-complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the b-galactosidase gene encoding an a-fragment of b-galactosidase.

 

SALES SPECIFICATION

APPEARANCE

white crystalline powder

ASSAY

99.0% min

MELTING POINT

230 C

OPTICAL ROTATION

-63° ~ -65° (c=1, DMF/water 1:1)

WATER

0.5% max

 

TRANSPORT & REGULATORY INFORMATION

UN NO.

 

HAZARD CLASS

 

PACKING GROUP  

 

SAFETY INFORMATION

HAZARD OVERVIEW

GHS (Globally Harmonised System) Classification: Not a dangerous substance. Potential Health Effects: Eyes - May cause eye irritation. Skin - May be harmful if absorbed through skin May cause skin irritation. Inhalation - May be harmful if inhaled. May cause respiratory tract irritation. Ingestion - May be harmful if swallowed.

HAZARD CODES

 

RISK PHRASES

 

SAFETY PHRASES

 

 

PACKING

Preserved in light-resistant and well-closed bottles

 

PRICE INFORMATION